A novel human leukocyte interferon cDNA clone (LeIF B) was identified in a cDNA library prepared using polyadenylated mRNA of a myeloblastoid cell line. The nucleotide sequence of LeIF B differs significantly from other published leukocyte interferon cDNA sequences. An expression plasmid was constructed which directs the synthesis in E. coli of 8 x 10(7) interferon units per liter of culture. LeIF B exhibits markedly different specificities from another bacterially synthesized human leukocyte interferon, LeIF A.
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rdfs:comment |
A novel human leukocyte interferon cDNA clone (LeIF B) was identified in a cDNA library prepared using polyadenylated mRNA of a myeloblastoid cell line. The nucleotide sequence of LeIF B differs significantly from other published leukocyte interferon cDNA sequences. An expression plasmid was constructed which directs the synthesis in E. coli of 8 x 10(7) interferon units per liter of culture. LeIF B exhibits markedly different specificities from another bacterially synthesized human leukocyte interferon, LeIF A.
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skos:exactMatch | |
uniprot:name |
Nucleic Acids Res.
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uniprot:author |
Goeddel D.V.,
Gray P.W.,
Leung D.,
Weck P.,
Yelverton E.
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uniprot:date |
1981
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uniprot:pages |
731-741
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uniprot:title |
Bacterial synthesis of a novel human leukocyte interferon.
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uniprot:volume |
9
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dc-term:identifier |
doi:10.1093/nar/9.3.731
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