Proc. Natl. Acad. Sci. U.S.A.

The S1 genes of the three serotypes of reovirus have been cloned and sequenced. The S1 genes encode protein sigma 1, the protein against which serotype-specific neutralizing antibodies are directed; it is also the reovirus hemagglutinin and cell-attachment protein and is a major determinant of host range/tissue specificity and of the nature of the interaction of reovirus with cells of the immune system. The S1 genes of serotypes 1, 2, and 3 are 1458, 1442, and 1416 nucleotides long, respectively. They possess untranslated regions 13, 13, and 12 nucleotides long at their 5' termini and 188, 229, and 36 nucleotides long at their 3' termini. They possess two open reading frames. The first starts with a "weak" initiation codon and extends for 418, 399, and 455 codons, respectively; this is the size expected for the sigma 1 proteins. The other reading frame starts at a "strong" initiation codon about 70 residues downstream from the 5' terminus but extends for only about 120 codons, being terminated by 3 in-phase termination codons in all three genes. The proteins encoded by these short open reading frames are quite basic. The serotype 1 and 2 S1 genes are much more closely related to each other (28% homology) than to the serotype 3 S1 gene (5% and 9% homology, respectively). These figures are based on direct homology calculations, adjusted for 25% random coincidence. Serologic evidence and hydrophobicity profiles agree that the sigma 1 proteins of serotypes 1 and 2 are much more closely related to each other (about 40% homology) than to that of serotype 3 (only about 20% homology). The fact that the serotype 1 and 2 S1 genes are much more closely related to each other than to the serotype 3 S1 gene is remarkable since for all other nine reovirus genes the serotype 1 and 3 genes are much more closely related to each other than to the serotype 2 gene. Mechanisms that may effect this remarkable evolutionary pattern are discussed.

Source:http://purl.uniprot.org/citations/3855545