DNA from monkey B-lymphotropic papovavirus (LPV) adapted to growth in human B-lymphoblastoid cell line BJA-B consisted of three classes of molecules of 5.1, 5.0, and 4.9 kilobases (kb), and the entire DNA sequence of 5.1-kb molecule was determined. The three types of LPV DNA yielded infectious virus upon transfection to BJA-B cells. Comparison of the structures among these clones and clone K38 sequenced by Pawlita et al. (1985) suggests that the transcriptional control region, the small-T antigen gene, and the VP-1 gene are variable in the LPV genome, and that the 5.0-kb LPV resembles the parent virus, from which all the others had evolved by duplication and deletion during passages in BJA-B cells, probably for adaptation to better growth in human cells.
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| rdfs:comment |
DNA from monkey B-lymphotropic papovavirus (LPV) adapted to growth in human B-lymphoblastoid cell line BJA-B consisted of three classes of molecules of 5.1, 5.0, and 4.9 kilobases (kb), and the entire DNA sequence of 5.1-kb molecule was determined. The three types of LPV DNA yielded infectious virus upon transfection to BJA-B cells. Comparison of the structures among these clones and clone K38 sequenced by Pawlita et al. (1985) suggests that the transcriptional control region, the small-T antigen gene, and the VP-1 gene are variable in the LPV genome, and that the 5.0-kb LPV resembles the parent virus, from which all the others had evolved by duplication and deletion during passages in BJA-B cells, probably for adaptation to better growth in human cells.
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| skos:exactMatch | |
| uniprot:name |
Jpn. J. Med. Sci. Biol.
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| uniprot:author |
Furuno A.,
Kanda T.,
Yoshiike K.
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| uniprot:date |
1986
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| uniprot:pages |
151-161
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| uniprot:title |
Monkey B-lymphotropic papovavirus genome: the entire DNA sequence and variable regions.
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| uniprot:volume |
39
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