Nucleic Acids Res.

The gam gene of bacteriophage Mu encodes a protein which protects linear double stranded DNA from exonuclease degradation in vitro and in vivo. We purified the Mu gam gene product to apparent homogeneity from cells in which it is over-produced from a plasmid clone. The purified protein is a dimer of identical subunits of 18.9 kd. It can aggregate DNA into large, rapidly sedimenting complexes and is a potent exonuclease inhibitor when bound to DNA. The N-terminal amino acid sequence of the purified protein was determined by automated degradation and the nucleotide sequence of the Mu gam gene is presented to accurately map its position in the Mu genome.

Source:http://purl.uniprot.org/citations/2945162

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
rdfs:comment
The gam gene of bacteriophage Mu encodes a protein which protects linear double stranded DNA from exonuclease degradation in vitro and in vivo. We purified the Mu gam gene product to apparent homogeneity from cells in which it is over-produced from a plasmid clone. The purified protein is a dimer of identical subunits of 18.9 kd. It can aggregate DNA into large, rapidly sedimenting complexes and is a potent exonuclease inhibitor when bound to DNA. The N-terminal amino acid sequence of the purified protein was determined by automated degradation and the nucleotide sequence of the Mu gam gene is presented to accurately map its position in the Mu genome.
skos:exactMatch
uniprot:name
Nucleic Acids Res.
uniprot:author
Akroyd J.E., Clayson E., Higgins N.P.
uniprot:date
1986
uniprot:pages
6901-6914
uniprot:title
Purification of the gam gene-product of bacteriophage Mu and determination of the nucleotide sequence of the gam gene.
uniprot:volume
14
dc-term:identifier
doi:10.1093/nar/14.17.6901