Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that were expressed in Escherichia coli from this determinant under the control of a phage T7 promoter were assigned to four open reading frames identified in the nucleotide sequence of the 6881-base-pair fragment containing the czc putative operon. The contributions of the polypeptides to the cation efflux system were analyzed with deletion derivatives of the 6.9-kilobase fragment, constructed, and expressed in E. coli under the control of the phage T7 promoter and in A. eutrophus under the control of the lac promoter.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that were expressed in Escherichia coli from this determinant under the control of a phage T7 promoter were assigned to four open reading frames identified in the nucleotide sequence of the 6881-base-pair fragment containing the czc putative operon. The contributions of the polypeptides to the cation efflux system were analyzed with deletion derivatives of the 6.9-kilobase fragment, constructed, and expressed in E. coli under the control of the phage T7 promoter and in A. eutrophus under the control of the lac promoter.
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| skos:exactMatch | |
| uniprot:name |
Proc. Natl. Acad. Sci. U.S.A.
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| uniprot:author |
Chu L.,
Nies A.,
Nies D.H.,
Silver S.
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| uniprot:date |
1989
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| uniprot:pages |
7351-7355
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| uniprot:title |
Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.
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| uniprot:volume |
86
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| dc-term:identifier |
doi:10.1073/pnas.86.19.7351
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