The effects of growth hormone (GH) on two constitutively expressed rat liver cytochrome P-450 (P-450) enzymes, namely P-450f (IIC7) and P-450PB1 (IIC6), have been investigated. Hypophysectomy of both male and female rats results in low expression of P-450f. Single daily injections of GH cannot restore expression of P-450f but further repress it. Continuous administration of GH, on the other hand, increases expression of P-450f to levels comparable to those of the normal male and female rat. P-450PB1 is minimally affected by hypophysectomy or GH treatment, although a weak but significant repression by continuous administration of GH can be detected. Run-on analysis shows that continuous GH treatment increases transcription of P-450f. In addition, the sequence of the 5' flanking region of this gene reveals DNA segments that might have a putative role in the transcriptional regulation of P-450f by GH.
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| rdfs:comment |
The effects of growth hormone (GH) on two constitutively expressed rat liver cytochrome P-450 (P-450) enzymes, namely P-450f (IIC7) and P-450PB1 (IIC6), have been investigated. Hypophysectomy of both male and female rats results in low expression of P-450f. Single daily injections of GH cannot restore expression of P-450f but further repress it. Continuous administration of GH, on the other hand, increases expression of P-450f to levels comparable to those of the normal male and female rat. P-450PB1 is minimally affected by hypophysectomy or GH treatment, although a weak but significant repression by continuous administration of GH can be detected. Run-on analysis shows that continuous GH treatment increases transcription of P-450f. In addition, the sequence of the 5' flanking region of this gene reveals DNA segments that might have a putative role in the transcriptional regulation of P-450f by GH.
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| skos:exactMatch | |
| uniprot:name |
Mol. Pharmacol.
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| uniprot:author |
Gustafsson J.-A.,
Stroem A.,
Westin S.,
Zaphiropoulos P.G.
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| uniprot:date |
1990
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| uniprot:pages |
192-197
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| uniprot:title |
Growth hormone regulation of the cytochrome P-450IIC subfamily in the rat: inductive, repressive, and transcriptional effects on P-450f (IIC7) and P-450PB1 (IIC6) gene expression.
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| uniprot:volume |
38
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