We have determined the sequence of a DNA fragment encoding the ADE2 gene from Saccharomyces cerevisiae. A DNA fragment of 2241 bp capable of complementing ade2 mutations was modified so it is available as a single BglII fragment for use in yeast vectors or for gene disruptions. The minimal fragment codes for a putative protein which is highly similar to the protein encoded by the ADE6 gene from Schizosaccharomyces pombe and to the proteins encoded by the purEK operon of Escherichia coli.
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rdfs:comment |
We have determined the sequence of a DNA fragment encoding the ADE2 gene from Saccharomyces cerevisiae. A DNA fragment of 2241 bp capable of complementing ade2 mutations was modified so it is available as a single BglII fragment for use in yeast vectors or for gene disruptions. The minimal fragment codes for a putative protein which is highly similar to the protein encoded by the ADE6 gene from Schizosaccharomyces pombe and to the proteins encoded by the purEK operon of Escherichia coli.
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skos:exactMatch | |
uniprot:name |
Gene
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uniprot:author |
Linder P.,
Stotz A.
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uniprot:date |
1990
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uniprot:pages |
91-98
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uniprot:title |
The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors.
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uniprot:volume |
95
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dc-term:identifier |
doi:10.1016/0378-1119(90)90418-Q
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