We show that PIF/activin can induce the formation of axial structures including a full-length notochord, segmented somites, and a neural tube in isolated epiblasts from chick blastulae. Using degenerate PCR primers, we have cloned a fragment of the activin beta B chain from chick hypoblast cDNA, and a fragment of the activin beta A chain from chick genomic DNA. Furthermore, we show that in the chick, activin is transcribed precisely when axial mesoderm is being induced. Since exogenous PIF/activin can induce the formation of axial structures and since activin beta B is transcribed at the time and place where the mesodermal axial structures are being induced, we propose that in the chick, activin B is the endogenous inducer of the body axis.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
We show that PIF/activin can induce the formation of axial structures including a full-length notochord, segmented somites, and a neural tube in isolated epiblasts from chick blastulae. Using degenerate PCR primers, we have cloned a fragment of the activin beta B chain from chick hypoblast cDNA, and a fragment of the activin beta A chain from chick genomic DNA. Furthermore, we show that in the chick, activin is transcribed precisely when axial mesoderm is being induced. Since exogenous PIF/activin can induce the formation of axial structures and since activin beta B is transcribed at the time and place where the mesodermal axial structures are being induced, we propose that in the chick, activin B is the endogenous inducer of the body axis.
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| skos:exactMatch | |
| uniprot:name |
Cell
|
| uniprot:author |
Bril A.,
Melton D.A.,
Mitrani E.,
Shimoni Y.,
Thomsen G.,
Ziv T.
|
| uniprot:date |
1990
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| uniprot:pages |
495-501
|
| uniprot:title |
Activin can induce the formation of axial structures and is expressed in the hypoblast of the chick.
|
| uniprot:volume |
63
|
| dc-term:identifier |
doi:10.1016/0092-8674(90)90446-L
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