The first transposable element to be isolated from Streptomyces fradiae, Tn4556, was completely sequenced; the total of 6625 bp have an overall G + C composition of 68%. Computer-aided analysis of this sequence reveals the location of nine open reading frames (ORFs). Several of these ORFs, numbers 1, 2, and 7, contain ribosome-binding sites (RBS) near their putative translation-initiation sites, which share identity with the consensus RBS sequences of Escherichia coli and Bacillus subtilis. ORF1 potentially encodes an 892-amino acid (aa) protein and this deduced aa sequence shares 61% identity with that of the transposase encoded by the tnpA gene of Tn3. Three other ORFs, 2, 3 and 5, potentially encode proteins which are similar in size to the resolvase protein encoded by the Tn3 gene tnpR; however, none of the protein products deduced from these ORF share extensive aa sequence identity with other resolvase proteins.
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| rdf:type | |
| rdfs:comment |
The first transposable element to be isolated from Streptomyces fradiae, Tn4556, was completely sequenced; the total of 6625 bp have an overall G + C composition of 68%. Computer-aided analysis of this sequence reveals the location of nine open reading frames (ORFs). Several of these ORFs, numbers 1, 2, and 7, contain ribosome-binding sites (RBS) near their putative translation-initiation sites, which share identity with the consensus RBS sequences of Escherichia coli and Bacillus subtilis. ORF1 potentially encodes an 892-amino acid (aa) protein and this deduced aa sequence shares 61% identity with that of the transposase encoded by the tnpA gene of Tn3. Three other ORFs, 2, 3 and 5, potentially encode proteins which are similar in size to the resolvase protein encoded by the Tn3 gene tnpR; however, none of the protein products deduced from these ORF share extensive aa sequence identity with other resolvase proteins.
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| skos:exactMatch | |
| uniprot:name |
Gene
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| uniprot:author |
Chung S.T.,
Siemieniak D.R.,
Slightom J.L.
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| uniprot:date |
1990
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| uniprot:pages |
1-9
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| uniprot:title |
Nucleotide sequence of Streptomyces fradiae transposable element Tn4556: a class-II transposon related to Tn3.
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| uniprot:volume |
86
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| dc-term:identifier |
doi:10.1016/0378-1119(90)90107-3
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