To identify islet amyloid polypeptide (IAPP) present in normal human pancreas, we isolated the peptide from a soluble peptide fraction of amyloid deposit-free pancreata of two non-diabetic patients by using reverse-phase high performance liquid chromatography coupled with a radioimmunoassay specific for human IAPP. IAPP(1-37) and IAPP(17-37) were isolated and their complete amino acid sequences were determined up to the C-terminus. Identification of IAPP in normal human pancreas suggests the possible biological function of IAPP as a novel pancreatic hormone in humans.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
To identify islet amyloid polypeptide (IAPP) present in normal human pancreas, we isolated the peptide from a soluble peptide fraction of amyloid deposit-free pancreata of two non-diabetic patients by using reverse-phase high performance liquid chromatography coupled with a radioimmunoassay specific for human IAPP. IAPP(1-37) and IAPP(17-37) were isolated and their complete amino acid sequences were determined up to the C-terminus. Identification of IAPP in normal human pancreas suggests the possible biological function of IAPP as a novel pancreatic hormone in humans.
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| skos:exactMatch | |
| uniprot:name |
Regul. Pept.
|
| uniprot:author |
Asai J.,
Kangawa K.,
Matsukura S.,
Matsuo H.,
Miyazato M.,
Nakazato M.
|
| uniprot:date |
1990
|
| uniprot:pages |
179-186
|
| uniprot:title |
Isolation and identification of islet amyloid polypeptide in normal human pancreas.
|
| uniprot:volume |
31
|
| dc-term:identifier |
doi:10.1016/0167-0115(90)90004-G
|