In our previous study, a novel P[8] subtype, i.e., P[8]b was identified for human rotavirus strains MMC38 and MMC71 detected in Bangladesh, of which the P types could not be determined by conventional RT-PCR genotyping methods. In the present study, a modified multiplex RT-PCR method was developed to detect P[8]b as well as common human rotavirus P types. With this method, P[8]b was detected in three strains among the 26 rotavirus specimens which had been judged as mixed P types in the previous study in Bangladesh. The VP4 nucleotide sequences of these strains showed more than 98.9% identities to those of strains MMC38 and MMC71. The newly designed RT-PCR method was considered as useful for identifying P[8]b and avoiding misclassification by the conventional RT-PCR genotyping methods.
| Predicate | Object |
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| rdf:type | |
| rdfs:comment |
In our previous study, a novel P[8] subtype, i.e., P[8]b was identified for human rotavirus strains MMC38 and MMC71 detected in Bangladesh, of which the P types could not be determined by conventional RT-PCR genotyping methods. In the present study, a modified multiplex RT-PCR method was developed to detect P[8]b as well as common human rotavirus P types. With this method, P[8]b was detected in three strains among the 26 rotavirus specimens which had been judged as mixed P types in the previous study in Bangladesh. The VP4 nucleotide sequences of these strains showed more than 98.9% identities to those of strains MMC38 and MMC71. The newly designed RT-PCR method was considered as useful for identifying P[8]b and avoiding misclassification by the conventional RT-PCR genotyping methods.
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| skos:exactMatch | |
| uniprot:name |
Jpn. J. Infect. Dis.
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| uniprot:author |
Chawla-Sarkar M.,
Ghosh S.,
Hossain M.A.,
Kobayashi N.,
Krishnan T.,
Nagashima S.,
Paul S.K.
|
| uniprot:date |
2010
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| uniprot:pages |
208-211
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| uniprot:title |
Identification of P[8]b subtype in OP354-like human rotavirus strains by a modified RT-PCR method.
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| uniprot:volume |
63
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