Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.
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| skos:exactMatch | |
| uniprot:name |
Gene
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| uniprot:author |
Cundliffe E.,
Financsek I.,
Kelemen G.H.
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| uniprot:date |
1991
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| uniprot:pages |
53-60
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| uniprot:title |
Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea.
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| uniprot:volume |
98
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| dc-term:identifier |
doi:10.1016/0378-1119(91)90103-I
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