F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH.
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| skos:exactMatch | |
| uniprot:name |
J. Bacteriol.
|
| uniprot:author |
Arenson B.,
Arutyunov D.,
Frost L.S.,
Manchak J.
|
| uniprot:date |
2010
|
| uniprot:pages |
1730-1734
|
| uniprot:title |
F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation.
|
| uniprot:volume |
192
|
| dc-term:identifier |
doi:10.1128/JB.00726-09
|