We have deduced the exon structure of the 5' untranslated region of the human cardiac alpha-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5' non-coding region of the alpha-myosin heavy chain gene is interrupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the alpha-myosin heavy chain promoter based on comparisons between the rat and human genes.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
We have deduced the exon structure of the 5' untranslated region of the human cardiac alpha-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5' non-coding region of the alpha-myosin heavy chain gene is interrupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the alpha-myosin heavy chain promoter based on comparisons between the rat and human genes.
|
| skos:exactMatch | |
| uniprot:name |
Biochem. Biophys. Res. Commun.
|
| uniprot:author |
Barton P.J.,
Brand N.J.,
Dabhade N.,
Yacoub M.
|
| uniprot:date |
1991
|
| uniprot:pages |
1255-1258
|
| uniprot:title |
Determination of the 5' exon structure of the human cardiac alpha-myosin heavy chain gene.
|
| uniprot:volume |
179
|
| dc-term:identifier |
doi:10.1016/0006-291X(91)91707-J
|