The gene encoding steroid inducible cytochrome P450 of Rhizopus nigricans ATCC 6227b has been found inside a HindIII fragment of the genomic DNA by hybridization with a partial length cDNA probe. The latter was isolated by immunoscreening a cDNA library prepared in the lambda gt11 expression system and identified on the basis of inducibility and sequence analysis. The nucleotide sequence of the cDNA probe revealed a coding sequence for the heme binding segment characteristic of the P450 gene family.
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| rdfs:comment |
The gene encoding steroid inducible cytochrome P450 of Rhizopus nigricans ATCC 6227b has been found inside a HindIII fragment of the genomic DNA by hybridization with a partial length cDNA probe. The latter was isolated by immunoscreening a cDNA library prepared in the lambda gt11 expression system and identified on the basis of inducibility and sequence analysis. The nucleotide sequence of the cDNA probe revealed a coding sequence for the heme binding segment characteristic of the P450 gene family.
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| skos:exactMatch | |
| uniprot:name |
Biochem. Biophys. Res. Commun.
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| uniprot:author |
Breskvar K.,
Cresnar B.,
Hudnik-Plevnik T.,
Plaper A.
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| uniprot:date |
1991
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| uniprot:pages |
1078-1083
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| uniprot:title |
Localization of the gene encoding steroid hydroxylase cytochrome P-450 from Rhizopus nigricans inside a HindIII fragment of genomic DNA.
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| uniprot:volume |
178
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| dc-term:identifier |
doi:10.1016/0006-291X(91)91002-T
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