The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.
|
| skos:exactMatch | |
| uniprot:name |
Science
|
| uniprot:author |
Goodwin S.S.,
Goshima G.,
Scholey J.M.,
Stuurman N.,
Vale R.D.,
Wollman R.,
Zhang N.
|
| uniprot:date |
2007
|
| uniprot:pages |
417-421
|
| uniprot:title |
Genes required for mitotic spindle assembly in Drosophila S2 cells.
|
| uniprot:volume |
316
|
| dc-term:identifier |
doi:10.1126/science.1141314
|