The death receptor 5 (DR-5), a receptor for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), is critical for TRAIL-mediated apoptosis in various tumor cells. The ESE-3, a member of Ets transcription factors, regulates the expression of a variety of cellular genes by binding to purine-rich GGAA/T core sequence in cooperation with other transcription factors and co-factors. In this study, we demonstrate for the first time that ESE-3 regulates DR-5 expression through Ets binding sequences on the DR-5 promoter. Using a combination of the electrophoretic mobility shift assay and the luciferase reporter assay, we identified putative Ets sites responsible for ESE-3 transcriptional activity on the DR-5 promoter. In addition, we show the possible involvement of co-factors CBP and p300 in ESE-3-mediated DR-5 up-regulation.
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| rdf:type | |
| rdfs:comment |
The death receptor 5 (DR-5), a receptor for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), is critical for TRAIL-mediated apoptosis in various tumor cells. The ESE-3, a member of Ets transcription factors, regulates the expression of a variety of cellular genes by binding to purine-rich GGAA/T core sequence in cooperation with other transcription factors and co-factors. In this study, we demonstrate for the first time that ESE-3 regulates DR-5 expression through Ets binding sequences on the DR-5 promoter. Using a combination of the electrophoretic mobility shift assay and the luciferase reporter assay, we identified putative Ets sites responsible for ESE-3 transcriptional activity on the DR-5 promoter. In addition, we show the possible involvement of co-factors CBP and p300 in ESE-3-mediated DR-5 up-regulation.
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| skos:exactMatch | |
| uniprot:name |
Biochem. Biophys. Res. Commun.
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| uniprot:author |
Cho J.-Y.,
Kwon T.K.,
Lim J.H.,
Park J.-W.,
Park Y.B.
|
| uniprot:date |
2006
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| uniprot:pages |
736-741
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| uniprot:title |
ESE-3 transcription factor is involved in the expression of death receptor (DR)-5 through putative Ets sites.
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| uniprot:volume |
350
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| dc-term:identifier |
doi:10.1016/j.bbrc.2006.09.102
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