The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.
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The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.
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| uniprot:name |
Science
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| uniprot:author |
Buchner E.,
Fouquet W.,
Heckmann M.,
Hell S.W.,
Kellner R.R.,
Kittel R.J.,
Pawlu C.,
Rasse T.M.,
Schmid A.,
Schmidt M.,
Sigrist S.J.,
Wagh D.A.,
Wichmann C.,
Willig K.I.
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| uniprot:date |
2006
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| uniprot:pages |
1051-1054
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| uniprot:title |
Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release.
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| uniprot:volume |
312
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| dc-term:identifier |
doi:10.1126/science.1126308
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