Here we propose the use of a 216-nucleotide fragment located in the carboxyl terminus of the E gene (E-COOH) and a pairwise-based comparison method for genotyping of dengue virus 1 (DENV-1) strains. We have applied this method to the detection and characterization of DENV-1 in serum samples from travelers returning from the tropics. The results obtained with the typing system correlate with the results obtained by comparison of the sequences of the entire E gene of the strains. The approach demonstrates utility in plotting the distribution and circulation of different genotypes of DENV-1 and also suggests the presence of two new clades of Indian strains. The integration of the method with an online database and a typing characterization tool enhances its strength. Additionally, the analysis of the complete E gene of DENV-1 strains suggested the occurrence of a nondescribed recombination event in the China GD23-95 strain. We propose the use of this methodology as a tool for real-time epidemiological surveillance of dengue virus infections and their pathogenesis.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
Here we propose the use of a 216-nucleotide fragment located in the carboxyl terminus of the E gene (E-COOH) and a pairwise-based comparison method for genotyping of dengue virus 1 (DENV-1) strains. We have applied this method to the detection and characterization of DENV-1 in serum samples from travelers returning from the tropics. The results obtained with the typing system correlate with the results obtained by comparison of the sequences of the entire E gene of the strains. The approach demonstrates utility in plotting the distribution and circulation of different genotypes of DENV-1 and also suggests the presence of two new clades of Indian strains. The integration of the method with an online database and a typing characterization tool enhances its strength. Additionally, the analysis of the complete E gene of DENV-1 strains suggested the occurrence of a nondescribed recombination event in the China GD23-95 strain. We propose the use of this methodology as a tool for real-time epidemiological surveillance of dengue virus infections and their pathogenesis.
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| skos:exactMatch | |
| uniprot:name |
J. Clin. Microbiol.
|
| uniprot:author |
Cabrerizo M.,
Domingo C.,
Gascon J.,
Jabado O.,
Lipkin W.I.,
Niedrig M.,
Palacios G.,
Reyes N.,
Tenorio A.
|
| uniprot:date |
2006
|
| uniprot:pages |
1519-1529
|
| uniprot:title |
Use of a short fragment of the C-terminal E gene for detection and characterization of two new lineages of dengue virus 1 in India.
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| uniprot:volume |
44
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| dc-term:identifier |
doi:10.1128/JCM.44.4.1519-1529.2006
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