This study developed a PCR-based mating-type assay for Clavicipitaceae. PCR primer sets for the mating-type genes MAT1-1-1 and MAT1-2-1 were designed based on the amino acid sequences of the conserved alpha and HMG boxes, respectively. The PCR-based mating-type assay could be applied for various clavicipitaceous genera (Balansia, Claviceps, Cordyceps and Epichloë). Most of the clavicipitaceous fungi possessed either MAT1-1-1 or MAT1-2-1, and were supposed to be heterothallic. Although the PCR products obtained by the mating-type assay were short, the phylogenetic trees of the mating-type genes gave better resolutions than that of 18S rDNA and agreed well to their econutritional modes.
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| rdf:type | |
| rdfs:comment |
This study developed a PCR-based mating-type assay for Clavicipitaceae. PCR primer sets for the mating-type genes MAT1-1-1 and MAT1-2-1 were designed based on the amino acid sequences of the conserved alpha and HMG boxes, respectively. The PCR-based mating-type assay could be applied for various clavicipitaceous genera (Balansia, Claviceps, Cordyceps and Epichloë). Most of the clavicipitaceous fungi possessed either MAT1-1-1 or MAT1-2-1, and were supposed to be heterothallic. Although the PCR products obtained by the mating-type assay were short, the phylogenetic trees of the mating-type genes gave better resolutions than that of 18S rDNA and agreed well to their econutritional modes.
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| skos:exactMatch | |
| uniprot:name |
FEMS Microbiol. Lett.
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| uniprot:author |
Hara A.,
Yamagishi K.,
Yokoyama E.
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| uniprot:date |
2004
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| uniprot:pages |
205-212
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| uniprot:title |
Development of a PCR-based mating-type assay for Clavicipitaceae.
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| uniprot:volume |
237
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| dc-term:identifier |
doi:10.1016/j.femsle.2004.06.033
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