Dopamine is an important neurotransmitter regulating light-dark adaptation in the retina. The effect of dopamine is widespread and dependent on the localization of its receptors. We performed single cell RT-PCR on A-type horizontal cells visually selected from dissociated rabbit retinal neurons. Dopamine D1-receptor mRNA was positively identified. Subsequent sequencing of the fragment showed 82% homology with rat D1 receptor and 87% homology with human D1 receptor. This study supported previous observations that dopamine regulated A-type horizontal cell coupling via D1 receptors at the level of transcription.
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Dopamine is an important neurotransmitter regulating light-dark adaptation in the retina. The effect of dopamine is widespread and dependent on the localization of its receptors. We performed single cell RT-PCR on A-type horizontal cells visually selected from dissociated rabbit retinal neurons. Dopamine D1-receptor mRNA was positively identified. Subsequent sequencing of the fragment showed 82% homology with rat D1 receptor and 87% homology with human D1 receptor. This study supported previous observations that dopamine regulated A-type horizontal cell coupling via D1 receptors at the level of transcription.
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| uniprot:name |
Neurosci. Lett.
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| uniprot:author |
Gaughwin P.,
He S.,
Li H.,
Li N.
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| uniprot:date |
2004
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| uniprot:pages |
146-148
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| uniprot:title |
Localization of dopamine D1-receptor to A-type horizontal cells in the rabbit retina by single cell RT-PCR.
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| uniprot:volume |
355
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| dc-term:identifier |
doi:10.1016/j.neulet.2003.10.042
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