Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.
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| skos:exactMatch | |
| uniprot:name |
Science
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| uniprot:author |
Akiyama K.,
Ansari Y.,
Arakawa T.,
Banh J.,
Banno F.,
Bowser L.,
Brooks S.Y.,
Carninci P.,
Chan M.M.,
Chang C.H.,
Chao Q.,
Chen H.,
Cheuk R.F.,
Choy N.,
Dale J.M.,
Davis R.W.,
Deng J.M.,
Ecker J.R.,
Enju A.,
Goldsmith A.D.,
Gurjal M.,
Hansen N.F.,
Hayashizaki Y.,
Hsuan V.W.,
Iida K.,
Ishida J.,
Jiang P.X.,
Johnson-Hopson C.,
Jones T.,
Kamiya A.,
Karlin-Newmann G.,
Karnes M.,
Kawai J.,
Khan S.,
Kim C.J.,
Koesema E.,
Lam B.,
Lee J.M.,
Lim J.,
Liu S.X.,
Meyers C.,
Miranda M.,
Nakajima M.,
Narusaka M.,
Nguyen M.,
Onodera C.S.,
Palm C.J.,
Pham P.K.,
Quach H.L.,
Sakano H.,
Sakurai T.,
Satou M.,
Seki M.,
Shinn P.,
Shinozaki K.,
Southwick A.M.,
Tamse R.,
Tang C.C.,
Theologis A.,
Toriumi M.J.,
Tripp M.,
Vaysberg M.,
Wallender E.K.,
Wong C.,
Wu H.C.,
Wu T.,
Yamada K.,
Yamamura Y.,
Yu G.,
Yuan S.
|
| uniprot:date |
2003
|
| uniprot:pages |
842-846
|
| uniprot:title |
Empirical analysis of transcriptional activity in the Arabidopsis genome.
|
| uniprot:volume |
302
|
| dc-term:identifier |
doi:10.1126/science.1088305
|