A new Arabidopsis meiotic mutant has been isolated. Homozygous ahp2-1 (Arabidopsis homologue pairing 2) plants were sterile because of failure of both male and female gametophyte development. Fluorescent in situ hybridisation showed that in ahp2-1 male meiocytes, chromosomes did not form bivalents during prophase I and instead seemed to associate indiscriminately. Chromosome fragmentation, chromatin bridges and unbalanced segregation were seen in anaphase I and anaphase II. The ahp2-1 mutation was caused by a T-DNA insertion in an Arabidopsis homologue of meu13+, which has been implicated in homologous chromosome pairing during meiosis in Schizosaccharomyces pombe. Our results suggest that meu13+ function is conserved in higher eukaryotes and support the idea that Arabidopsis, yeast and mouse share a pairing pathway that is not present in Drosophila melanogaster and Caenorhabditis elegans.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
A new Arabidopsis meiotic mutant has been isolated. Homozygous ahp2-1 (Arabidopsis homologue pairing 2) plants were sterile because of failure of both male and female gametophyte development. Fluorescent in situ hybridisation showed that in ahp2-1 male meiocytes, chromosomes did not form bivalents during prophase I and instead seemed to associate indiscriminately. Chromosome fragmentation, chromatin bridges and unbalanced segregation were seen in anaphase I and anaphase II. The ahp2-1 mutation was caused by a T-DNA insertion in an Arabidopsis homologue of meu13+, which has been implicated in homologous chromosome pairing during meiosis in Schizosaccharomyces pombe. Our results suggest that meu13+ function is conserved in higher eukaryotes and support the idea that Arabidopsis, yeast and mouse share a pairing pathway that is not present in Drosophila melanogaster and Caenorhabditis elegans.
|
| skos:exactMatch | |
| uniprot:name |
Plant J.
|
| uniprot:author |
Beven A.,
Lawrenson T.,
Sablowski R.,
Schommer C.,
Shaw P.
|
| uniprot:date |
2003
|
| uniprot:pages |
1-11
|
| uniprot:title |
AHP2 is required for bivalent formation and for segregation of homologous chromosomes in Arabidopsis meiosis.
|
| uniprot:volume |
36
|
| dc-term:identifier |
doi:10.1046/j.1365-313X.2003.01850.x
|