A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.
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| skos:exactMatch | |
| uniprot:name |
DNA Seq.
|
| uniprot:author |
Bergstroem S.,
Hansson L.,
Hernell O.,
Loennerdal B.,
Nilsson A.K.,
Stroemqvist M.
|
| uniprot:date |
1992
|
| uniprot:pages |
245-246
|
| uniprot:title |
Cloning and sequencing of human kappa-casein cDNA.
|
| uniprot:volume |
3
|