Four new members of the ERF (ethylene-response factor) family of plant-specific DNA-binding (GCC box) factors were isolated from tomato fruit (LeERF1-4). Phylogenetic analysis indicated that LeERF2 belongs to a new ERF class, characterized by a conserved N-terminal signature sequence. Expression patterns and cis/trans binding affinities differed between the LeERFs. Combining experimental data and modeled three-dimensional analysis, it was shown that binding affinity of the LeERFs was affected by both the variation of nucleotides surrounding the DNA cis-element sequence and the nature of critical amino acid residues within the ERF domain.
| Predicate | Object |
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| rdf:type | |
| rdfs:comment |
Four new members of the ERF (ethylene-response factor) family of plant-specific DNA-binding (GCC box) factors were isolated from tomato fruit (LeERF1-4). Phylogenetic analysis indicated that LeERF2 belongs to a new ERF class, characterized by a conserved N-terminal signature sequence. Expression patterns and cis/trans binding affinities differed between the LeERFs. Combining experimental data and modeled three-dimensional analysis, it was shown that binding affinity of the LeERFs was affected by both the variation of nucleotides surrounding the DNA cis-element sequence and the nature of critical amino acid residues within the ERF domain.
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| skos:exactMatch | |
| uniprot:name |
FEBS Lett.
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| uniprot:author |
Bouzayen M.,
Jones B.,
Latche A.,
Pech J.-C.,
Pesquet E.,
Regad F.,
Sanchez-Ballesta M.T.,
Tournier B.
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| uniprot:date |
2003
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| uniprot:pages |
149-154
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| uniprot:title |
New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element.
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| uniprot:volume |
550
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| dc-term:identifier |
doi:10.1016/S0014-5793(03)00757-9
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