The focus of this study was to determine the functional capacity of class II transactivator (CIITA), a regulatory factor of major histocompatibility complex (MHC) class II genes, in K-562 cells. We show that CIITA mRNA is present in K-562 cells and the interferon-gamma (IFN-gamma)-inducible CIITA promoter-IV exhibits low levels of basal activity, which is greatly enhanced upon treatment with IFN-gamma. Further study revealed that the CIITA cDNA contains an insertion of genomic sequence, which introduces a stop codon. The truncated coding region of the CIITA transcript in K-562 cells provides a possible explanation for the absence of MHC class II molecules.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The focus of this study was to determine the functional capacity of class II transactivator (CIITA), a regulatory factor of major histocompatibility complex (MHC) class II genes, in K-562 cells. We show that CIITA mRNA is present in K-562 cells and the interferon-gamma (IFN-gamma)-inducible CIITA promoter-IV exhibits low levels of basal activity, which is greatly enhanced upon treatment with IFN-gamma. Further study revealed that the CIITA cDNA contains an insertion of genomic sequence, which introduces a stop codon. The truncated coding region of the CIITA transcript in K-562 cells provides a possible explanation for the absence of MHC class II molecules.
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| skos:exactMatch | |
| uniprot:name |
Leuk. Res.
|
| uniprot:author |
Day N.E.,
Ichiki A.T.,
Ugai H.,
Yokoyama K.K.
|
| uniprot:date |
2003
|
| uniprot:pages |
1027-1038
|
| uniprot:title |
K-562 cells lack MHC class II expression due to an alternatively spliced CIITA transcript with a truncated coding region.
|
| uniprot:volume |
27
|
| dc-term:identifier |
doi:10.1016/S0145-2126(03)00072-9
|