A platelet agglutinating inhibiting protein (agkisacutacin) was isolated from the venom of Agkistrodon acutus by DEAE Sepharose Fast Flow and size exclusion chromatography. The purified product was a 29 kD protein composed of two disulfide bond-linked polypeptide chains of molecular weight of 14 kD, 15 kD, respectively. It completely inhibited ristocetin-induced platelet agglutination with an IC(50) value of 18.5 mg/L. It also inhibited thrombin-induced platelet aggregation with an IC(50) value of 1.22 g/L, but it did not affect the platelet aggregation induced by collagen and ADP. No fibrinolytic activity, phospholipase A(2) activity, anticoagulant activity, haemorrhagic activity or lethal activity were detected in agkisacutacin. Therefore this protein may offer considerable therapeutic potential in treatment of platelet-rich thrombosis.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
A platelet agglutinating inhibiting protein (agkisacutacin) was isolated from the venom of Agkistrodon acutus by DEAE Sepharose Fast Flow and size exclusion chromatography. The purified product was a 29 kD protein composed of two disulfide bond-linked polypeptide chains of molecular weight of 14 kD, 15 kD, respectively. It completely inhibited ristocetin-induced platelet agglutination with an IC(50) value of 18.5 mg/L. It also inhibited thrombin-induced platelet aggregation with an IC(50) value of 1.22 g/L, but it did not affect the platelet aggregation induced by collagen and ADP. No fibrinolytic activity, phospholipase A(2) activity, anticoagulant activity, haemorrhagic activity or lethal activity were detected in agkisacutacin. Therefore this protein may offer considerable therapeutic potential in treatment of platelet-rich thrombosis.
|
| skos:exactMatch | |
| uniprot:name |
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao
|
| uniprot:author |
Cheng X.,
Li X.-M.,
Li X.Y.,
Liu J.,
Liu Q.D.,
Xu Z.Y.
|
| uniprot:date |
2000
|
| uniprot:pages |
653-656
|
| uniprot:title |
Purification and characterization of a platelet agglutinating inhibiting protein (Agkisacutacin) from Agkistrodon acutus venom.
|
| uniprot:volume |
32
|