We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the approximately 800 inner-membrane proteins thought to exist in E. coli.
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| rdf:type | |
| rdfs:comment |
We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the approximately 800 inner-membrane proteins thought to exist in E. coli.
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| skos:exactMatch | |
| uniprot:name |
Proc. Natl. Acad. Sci. U.S.A.
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| uniprot:author |
Chin C.-N.,
Drew D.,
Nilsson J.,
Sjoestrand D.,
Urbig T.,
de Gier J.-W.,
von Heijne G.
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| uniprot:date |
2002
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| uniprot:pages |
2690-2695
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| uniprot:title |
Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis.
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| uniprot:volume |
99
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| dc-term:identifier |
doi:10.1073/pnas.052018199
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