The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A DeltahupSL deletion mutant of Mc. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo.
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The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A DeltahupSL deletion mutant of Mc. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo.
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| uniprot:name |
FEMS Microbiol. Lett.
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| uniprot:author |
Bodrossy L.,
Csaki R.,
Hanczar T.,
Kovacs K.L.,
Murrell J.C.
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| uniprot:date |
2001
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| uniprot:pages |
203-207
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| uniprot:title |
Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath).
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| uniprot:volume |
205
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