Human lactoferrin (hLF) cDNA was amplified by RT-PCR from normal human mammary tissue obtained from Daxing County of Beijing City of China, and then subcloned into pGEM-T vector. hLF cDNA sequence was determined, which consists of 2136 bp. Comparison with five other hLF cDNA sequences registered in GenBank shows 99% homology in DNA sequence. However, there are two base substitutions (nucleotide 1740 G-->C, nucleotide 1756 T-->C), one of which subsequently leads to an amino acid change (residue 580 Glu-->Asp).
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|---|---|
| rdf:type | |
| rdfs:comment |
Human lactoferrin (hLF) cDNA was amplified by RT-PCR from normal human mammary tissue obtained from Daxing County of Beijing City of China, and then subcloned into pGEM-T vector. hLF cDNA sequence was determined, which consists of 2136 bp. Comparison with five other hLF cDNA sequences registered in GenBank shows 99% homology in DNA sequence. However, there are two base substitutions (nucleotide 1740 G-->C, nucleotide 1756 T-->C), one of which subsequently leads to an amino acid change (residue 580 Glu-->Asp).
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| skos:exactMatch | |
| uniprot:name |
Sheng Wu Gong Cheng Xue Bao
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| uniprot:author |
Chen X.Z.,
Cheng H.,
Huan L.D.
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| uniprot:date |
2001
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| uniprot:pages |
385-387
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| uniprot:title |
cDNA cloning and sequence analysis of human lactoferrin.
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| uniprot:volume |
17
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