Although auxin is known to regulate many processes in plant development and has been studied for over a century, the mechanisms whereby plants produce it have remained elusive. Here we report the characterization of a dominant Arabidopsis mutant, yucca, which contains elevated levels of free auxin. YUCCA encodes a flavin monooxygenase-like enzyme and belongs to a family that includes at least nine other homologous Arabidopsis genes, a subset of which appears to have redundant functions. Results from tryptophan analog feeding experiments and biochemical assays indicate that YUCCA catalyzes hydroxylation of the amino group of tryptamine, a rate-limiting step in tryptophan-dependent auxin biosynthesis.
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| rdfs:comment |
Although auxin is known to regulate many processes in plant development and has been studied for over a century, the mechanisms whereby plants produce it have remained elusive. Here we report the characterization of a dominant Arabidopsis mutant, yucca, which contains elevated levels of free auxin. YUCCA encodes a flavin monooxygenase-like enzyme and belongs to a family that includes at least nine other homologous Arabidopsis genes, a subset of which appears to have redundant functions. Results from tryptophan analog feeding experiments and biochemical assays indicate that YUCCA catalyzes hydroxylation of the amino group of tryptamine, a rate-limiting step in tryptophan-dependent auxin biosynthesis.
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| uniprot:name |
Science
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| uniprot:author |
Cashman J.R.,
Chory J.,
Christensen S.K.,
Cohen J.D.,
Fankhauser C.,
Weigel D.,
Zhao Y.
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| uniprot:date |
2001
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| uniprot:pages |
306-309
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| uniprot:title |
A role for flavin monooxygenase-like enzymes in auxin biosynthesis.
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| uniprot:volume |
291
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| dc-term:identifier |
doi:10.1126/science.291.5502.306
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