A novel gene for advanced red-shifted protein with an emission maximum at 593 nm was cloned from Discosoma coral. The protein, named dsFP593, is highly homologous to the recently described GFP-like protein drFP583 with an emission maximum at 583 nm. Using the remarkable similarity of the drFP583 and dsFP593 genes, we performed a 'shuffling' procedure to generate a pool of mutants consisting of various combinations of parts of both genes. One 'hybrid gene' was chosen for subsequent random mutagenesis, which resulted in a mutant variant with a uniquely red-shifted emission maximum at 616 nm.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
A novel gene for advanced red-shifted protein with an emission maximum at 593 nm was cloned from Discosoma coral. The protein, named dsFP593, is highly homologous to the recently described GFP-like protein drFP583 with an emission maximum at 583 nm. Using the remarkable similarity of the drFP583 and dsFP593 genes, we performed a 'shuffling' procedure to generate a pool of mutants consisting of various combinations of parts of both genes. One 'hybrid gene' was chosen for subsequent random mutagenesis, which resulted in a mutant variant with a uniquely red-shifted emission maximum at 616 nm.
|
| skos:exactMatch | |
| uniprot:name |
FEBS Lett.
|
| uniprot:author |
Barsova E.V.,
Chen Y.,
Ding L.,
Fradkov A.F.,
Lukyanov S.A.,
Matz M.V.
|
| uniprot:date |
2000
|
| uniprot:pages |
127-130
|
| uniprot:title |
Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence.
|
| uniprot:volume |
479
|
| dc-term:identifier |
doi:10.1016/S0014-5793(00)01895-0
|