Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.
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| skos:exactMatch | |
| uniprot:name |
J. Clin. Microbiol.
|
| uniprot:author |
Chaturvedi S.,
Chaturvedi V.,
Fan J.,
McClelland C.M.,
Rodeghier B.,
Wickes B.L.
|
| uniprot:date |
2000
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| uniprot:pages |
2007-2009
|
| uniprot:title |
Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies.
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| uniprot:volume |
38
|