We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/-DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering.
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| rdf:type | |
| rdfs:comment |
We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/-DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering.
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| skos:exactMatch | |
| uniprot:name |
Nucleic Acids Res.
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| uniprot:author |
Azzalin C.,
Brown W.,
Fukagawa T.,
Griffin D.,
Hayward N.,
Stewart A.F.,
Yang J.
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| uniprot:date |
1999
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| uniprot:pages |
1966-1969
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| uniprot:title |
The chicken HPRT gene: a counter selectable marker for the DT40 cell line.
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| uniprot:volume |
27
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| dc-term:identifier |
doi:10.1093/nar/27.9.1966
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