The goals of this study were to identify specific mRNA for isoforms of calmodulin-dependent protein kinase II in chicken forebrain, prepare a cDNA expression library, and perform a sequence analysis of the kinase cDNA. Specific mRNAs for alpha- and beta-subunits of the kinase were identified in Northern blots. The mRNA for the alpha-subunit is larger in the chicken that in the rat, and for the beta-subunit is smaller in the chicken than the rat. Nucleotide sequencing of selected clones demonstrated the presence of an alpha-subunit with a 33 nucleotide insert known as the alpha-B-isoform. Clones of the beta-subunit showed it to contain a deletion of six nucleotides relative to previously described sequences. Variability in the mRNAs of calmodulin kinase II, as shown here, reflect the presence of species-dependent variability in gene structure as well as the presence of different functional isoforms.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The goals of this study were to identify specific mRNA for isoforms of calmodulin-dependent protein kinase II in chicken forebrain, prepare a cDNA expression library, and perform a sequence analysis of the kinase cDNA. Specific mRNAs for alpha- and beta-subunits of the kinase were identified in Northern blots. The mRNA for the alpha-subunit is larger in the chicken that in the rat, and for the beta-subunit is smaller in the chicken than the rat. Nucleotide sequencing of selected clones demonstrated the presence of an alpha-subunit with a 33 nucleotide insert known as the alpha-B-isoform. Clones of the beta-subunit showed it to contain a deletion of six nucleotides relative to previously described sequences. Variability in the mRNAs of calmodulin kinase II, as shown here, reflect the presence of species-dependent variability in gene structure as well as the presence of different functional isoforms.
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| skos:exactMatch | |
| uniprot:name |
J. Mol. Neurosci.
|
| uniprot:author |
Cooper N.G.,
Gregg R.G.,
Li G.,
Liu N.,
Liu O.L.,
Mower G.D.
|
| uniprot:date |
1998
|
| uniprot:pages |
135-139
|
| uniprot:title |
Molecular cloning and analysis of Ca2+/calmodulin-dependent protein kinase II from the chicken brain.
|
| uniprot:volume |
11
|
| dc-term:identifier |
doi:10.1385/JMN:11:2:135
|