Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.
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| skos:exactMatch | |
| uniprot:name |
Mol. Cell
|
| uniprot:author |
Kingston R.E.,
Narlikar G.J.,
Phelan M.L.,
Sif S.
|
| uniprot:date |
1999
|
| uniprot:pages |
247-253
|
| uniprot:title |
Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.
|
| uniprot:volume |
3
|
| dc-term:identifier |
doi:10.1016/S1097-2765(00)80315-9
|