The enzyme is activated by S-adenosyl-methionine. Activity is dependent on the levels of Fe(2+), S(2-) and Co(2+). Activity is stimulated by addition of EDTA. S-adenosylhomocysteine competitively inhibits the activity whereas 5'-methylthioadenosine is not inhibitory in the presence of S-adenosylmethionine. Competitively inhibited by 4-thialysine. Inhibited by sodium borohydride (1 mM) when added with 2 mM dithionate. Moderately inhibited by beta-mercaptoethanol (30 mM) along with dithionate. Higher concentrations of Fe(2+) partially inhibit the activity and Co(2+) at 1mM is a strong inhibitor. Hydroxylamine, isonicotinic acid hydrazide inhibit effectively, in addition, hydrazine, D-penicillamine and D-cycloserine are also inhibitory at high concentrations.
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The enzyme is activated by S-adenosyl-methionine. Activity is dependent on the levels of Fe(2+), S(2-) and Co(2+). Activity is stimulated by addition of EDTA. S-adenosylhomocysteine competitively inhibits the activity whereas 5'-methylthioadenosine is not inhibitory in the presence of S-adenosylmethionine. Competitively inhibited by 4-thialysine. Inhibited by sodium borohydride (1 mM) when added with 2 mM dithionate. Moderately inhibited by beta-mercaptoethanol (30 mM) along with dithionate. Higher concentrations of Fe(2+) partially inhibit the activity and Co(2+) at 1mM is a strong inhibitor. Hydroxylamine, isonicotinic acid hydrazide inhibit effectively, in addition, hydrazine, D-penicillamine and D-cycloserine are also inhibitory at high concentrations.
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