The catalytic efficiency observed with HKHD as substrate is 750-fold higher with Ni(2+) as cofactor than that with Mg(2+) as cofactor. 4-hydroxy-2-keto-5-phenyl-pentanoate and 4-hydroxy-2-keto-6-phenylhexanoate are not substrates, suggesting a requirement for an aliphatic or less bulky distal end of the substrate.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The catalytic efficiency observed with HKHD as substrate is 750-fold higher with Ni(2+) as cofactor than that with Mg(2+) as cofactor. 4-hydroxy-2-keto-5-phenyl-pentanoate and 4-hydroxy-2-keto-6-phenylhexanoate are not substrates, suggesting a requirement for an aliphatic or less bulky distal end of the substrate.
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| uniprot:measuredAffinity |
0.078 mM for 2-keto-3-deoxy-L-rhamnonate (in the presence of magnesium),
0.14 mM for 2-keto-3-deoxy-L-mannonate (in the presence of magnesium),
0.8 mM for 2-keto-3-deoxy-L-lyxonate (in the presence of magnesium),
0.15 mM for 4-hydroxy-2-ketoheptane-1,7-dioate (in the presence of magnesium),
0.1 mM for 4-hydroxy-2-ketoheptane-1,7-dioate (in the presence of nickel),
0.1 mM for 4-hydroxy-2-ketopentanoate (in the presence of nickel),
0.05 mM for 4-hydroxy-2-ketohexanoate (in the presence of nickel)
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