Q08945

FUNCTION: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63. SUBUNIT: Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Binds to histone H3-H4 tetramers, but not to intact nucleosomes. Interacts with isoform gamma of TP63. Interacts with FYTTD1/UIF, SRF and NEK9. SUBCELLULAR LOCATION: Nucleus. Nucleus, nucleolus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci. DOMAIN: The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin. PTM: Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity. PTM: Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein. PTM: Sumoylated. MISCELLANEOUS: Autoantibodies against SSRP1 are present in sera from patients with systemic lupus erythematosus, but not other rheumatic diseases. SIMILARITY: Belongs to the SSRP1 family. SIMILARITY: Contains 1 HMG box DNA-binding domain. SEQUENCE CAUTION: Sequence=AAH91486.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; GENE SYNONYMS:SSRP1 FACT80. COPYRIGHT: Protein annotation is derived from the UniProt Consortium (http://www.uniprot.org/). Distributed under the Creative Commons Attribution-NoDerivs License., SEQUENCE 709 AA; 81075 MW; 4E7EE3735EB41082 CRC64;

SSRP1_HUMAN

urn:biopax:ModificationFeature:SSRP1_HUMAN_1, urn:biopax:ModificationFeature:SSRP1_HUMAN_10, urn:biopax:ModificationFeature:SSRP1_HUMAN_11, urn:biopax:ModificationFeature:SSRP1_HUMAN_12, urn:biopax:ModificationFeature:SSRP1_HUMAN_13, urn:biopax:ModificationFeature:SSRP1_HUMAN_14, urn:biopax:ModificationFeature:SSRP1_HUMAN_15, urn:biopax:ModificationFeature:SSRP1_HUMAN_16, urn:biopax:ModificationFeature:SSRP1_HUMAN_17, urn:biopax:ModificationFeature:SSRP1_HUMAN_18, urn:biopax:ModificationFeature:SSRP1_HUMAN_19, urn:biopax:ModificationFeature:SSRP1_HUMAN_2, urn:biopax:ModificationFeature:SSRP1_HUMAN_20, urn:biopax:ModificationFeature:SSRP1_HUMAN_21, urn:biopax:ModificationFeature:SSRP1_HUMAN_3, urn:biopax:ModificationFeature:SSRP1_HUMAN_4, urn:biopax:ModificationFeature:SSRP1_HUMAN_5, urn:biopax:ModificationFeature:SSRP1_HUMAN_6, urn:biopax:ModificationFeature:SSRP1_HUMAN_7, urn:biopax:ModificationFeature:SSRP1_HUMAN_8, urn:biopax:ModificationFeature:SSRP1_HUMAN_9

MAETLEFNDVYQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGHGLKLLTKNGHVYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCTTGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSGLLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE