| Predicate | Object |
|---|---|
| rdf:type | |
| biopax3:comment |
FUNCTION: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. FUNCTION: Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may downregulate eNOS activity by forming heterodimers with isoform 1. CATALYTIC ACTIVITY: L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+). COFACTOR: Heme group. COFACTOR: Binds 1 FAD. COFACTOR: Binds 1 FMN. COFACTOR: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme. ENZYME REGULATION: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN. SUBCELLULAR LOCATION: Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. ALTERNATIVE PRODUCTS: Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P29474-1; Sequence=Displayed; Name=eNOS13C; IsoId=P29474-2; Sequence=VSP_042625, VSP_042626; Note=Lacks eNOS activity; TISSUE SPECIFICITY: Platelets, placenta, liver and kidney. PTM: Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)- calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. POLYMORPHISM: Variation in NOS3 seem to be associated with susceptibility to coronary spasm. SIMILARITY: Belongs to the NOS family. SIMILARITY: Contains 1 FAD-binding FR-type domain. SIMILARITY: Contains 1 flavodoxin-like domain. WEB RESOURCE: Name=Wikipedia; Note=Nitric oxide synthase entry; URL="http://en.wikipedia.org/wiki/Nitric_oxide_synthase"; WEB RESOURCE: Name=GeneReviews; URL="http://www.ncbi.nlm.nih.gov/sites/GeneTests/lab/gene/NOS3"; WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/nos3/"; GENE SYNONYMS:NOS3. COPYRIGHT: Protein annotation is derived from the UniProt Consortium (http://www.uniprot.org/). Distributed under the Creative Commons Attribution-NoDerivs License.,
SEQUENCE 1203 AA; 133289 MW; CD9E60FA277B22C6 CRC64;
|
| biopax3:xref |
urn:biopax:RelationshipXref:HGNC_HGNC:7876,
urn:biopax:RelationshipXref:NCBI GENE_4846,
urn:biopax:RelationshipXref:REFSEQ_NP_000594,
urn:biopax:RelationshipXref:REFSEQ_NP_001153581,
urn:biopax:RelationshipXref:REFSEQ_NP_001153582,
urn:biopax:RelationshipXref:REFSEQ_NP_001153583,
urn:biopax:UnificationXref:UNIPROT_A0S0A8,
urn:biopax:UnificationXref:UNIPROT_A8KA63,
urn:biopax:UnificationXref:UNIPROT_B2RCQ1,
urn:biopax:UnificationXref:UNIPROT_P29474,
urn:biopax:UnificationXref:UNIPROT_Q13662,
urn:biopax:UnificationXref:UNIPROT_Q14251,
urn:biopax:UnificationXref:UNIPROT_Q14434,
urn:biopax:UnificationXref:UNIPROT_Q548C1,
urn:biopax:UnificationXref:UNIPROT_Q6GSL5,
urn:biopax:UnificationXref:UNIPROT_Q9UDC6
|
| biopax3:displayName |
NOS3_HUMAN
|
| biopax3:name |
1.14.13.39,
Constitutive NOS,
EC-NOS,
Endothelial NOS,
NOS type III,
NOS3,
NOSIII,
cNOS,
eNOS
|
| biopax3:entityFeature | |
| biopax3:organism | |
| biopax3:sequence |
MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP
|
| biopax3:standardName |
Nitric oxide synthase, endothelial
|