P28028

FUNCTION: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron (By similarity). CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Binds 2 zinc ions per subunit (Potential). SUBUNIT: Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr- 790. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH (By similarity). Interacts with PRMT5 (By similarity). SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Cell membrane (By similarity). Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes (By similarity). ALTERNATIVE PRODUCTS: Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P28028-1; Sequence=Displayed; Name=2; IsoId=P28028-2; Sequence=VSP_022169, VSP_022170; Note=No experimental confirmation available; PTM: Phosphorylation at Ser-348 by SGK1 inhibits its activity (By similarity). PTM: Methylation by PRMT5 decreases stability and kinase activity. DISEASE: Note=Participates in a chromosomal translocation that produces a Tif1a-BRAF (T18) oncogene originally isolated from a furfural-induced hepatoma. SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. SIMILARITY: Contains 1 phorbol-ester/DAG-type zinc finger. SIMILARITY: Contains 1 protein kinase domain. SIMILARITY: Contains 1 RBD (Ras-binding) domain. SEQUENCE CAUTION: Sequence=AAA37320.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=AAA37320.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA; GENE SYNONYMS:Braf B-raf. COPYRIGHT: Protein annotation is derived from the UniProt Consortium (http://www.uniprot.org/). Distributed under the Creative Commons Attribution-NoDerivs License., SEQUENCE 804 AA; 88780 MW; 4C6CC16A2C660A17 CRC64;

BRAF_MOUSE

urn:biopax:ModificationFeature:BRAF_MOUSE_1, urn:biopax:ModificationFeature:BRAF_MOUSE_10, urn:biopax:ModificationFeature:BRAF_MOUSE_11, urn:biopax:ModificationFeature:BRAF_MOUSE_12, urn:biopax:ModificationFeature:BRAF_MOUSE_13, urn:biopax:ModificationFeature:BRAF_MOUSE_14, urn:biopax:ModificationFeature:BRAF_MOUSE_15, urn:biopax:ModificationFeature:BRAF_MOUSE_16, urn:biopax:ModificationFeature:BRAF_MOUSE_17, urn:biopax:ModificationFeature:BRAF_MOUSE_2, urn:biopax:ModificationFeature:BRAF_MOUSE_3, urn:biopax:ModificationFeature:BRAF_MOUSE_4, urn:biopax:ModificationFeature:BRAF_MOUSE_5, urn:biopax:ModificationFeature:BRAF_MOUSE_6, urn:biopax:ModificationFeature:BRAF_MOUSE_7, urn:biopax:ModificationFeature:BRAF_MOUSE_8, urn:biopax:ModificationFeature:BRAF_MOUSE_9

MAALSGGGGSSSGGGGGGGGGGGGGDGGGGAEQGQALFNGDMEPEAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLVFQTPTDASRNNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPVPQEEASFPETALPSGSSSAPPSDSTGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDEKFPEVELQDQRDLIRDQGFRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSSSEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK