| Predicate | Object |
|---|---|
| rdf:type | |
| biopax3:comment |
FUNCTION: Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose- 5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. SUBUNIT: Heterodimer of a 70 kDa (XRCC6) and a 80 kDa (XRCC5) subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NAA15, and this complex binds to the osteocalcin promoter and activates osteocalcin expression. In addition, XRCC6 interacts with the osteoblast-specific transcription factors MSX2, RUNX2 and DLX5. Interacts with ELF3. Interacts with XRCC6BP1. The XRCC5/6 dimer associates in a DNA-dependent manner with APEX1. Interacts with CLU (By similarity). Binds to CDK9 isoform 2. SUBCELLULAR LOCATION: Nucleus. Chromosome. DEVELOPMENTAL STAGE: Expression does not increase during promyelocyte differentiation. INDUCTION: In osteoblasts, by FGF2. PTM: Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. MISCELLANEOUS: Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to XRCC5 and XRCC6. Existence of a major autoantigenic epitope or epitopes on the C-terminal 190 amino acids of XRCC6 containing the leucine repeat. The majority of autoantibodies to XRCC6 in most sera from patients with SLE seem to be reactive with this region. SIMILARITY: Belongs to the ku70 family. SIMILARITY: Contains 1 Ku domain. SIMILARITY: Contains 1 SAP domain. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/g22p1/"; GENE SYNONYMS:XRCC6 G22P1. COPYRIGHT: Protein annotation is derived from the UniProt Consortium (http://www.uniprot.org/). Distributed under the Creative Commons Attribution-NoDerivs License.,
SEQUENCE 609 AA; 69843 MW; BBD3CD434526DFCB CRC64;
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| biopax3:xref |
urn:biopax:RelationshipXref:HGNC_HGNC:4055,
urn:biopax:RelationshipXref:NCBI GENE_2547,
urn:biopax:RelationshipXref:REFSEQ_NP_001460,
urn:biopax:UnificationXref:UNIPROT_P12956,
urn:biopax:UnificationXref:UNIPROT_Q6FG89,
urn:biopax:UnificationXref:UNIPROT_Q9UCQ2,
urn:biopax:UnificationXref:UNIPROT_Q9UCQ3
|
| biopax3:displayName |
XRCC6_HUMAN
|
| biopax3:name |
3.6.4.-,
4.2.99.-,
5'-dRP lyase Ku70,
5'-deoxyribose-5-phosphate lyase Ku70,
70 kDa subunit of Ku antigen,
ATP-dependent DNA helicase 2 subunit 1,
ATP-dependent DNA helicase II 70 kDa subunit,
CTC box-binding factor 75 kDa subunit,
CTC75,
CTCBF,
DNA repair protein XRCC6,
Ku70,
Lupus Ku autoantigen protein p70,
TLAA,
Thyroid-lupus autoantigen,
X-ray repair complementing defective repair in Chinese hamster cells 6,
XRCC6
|
| biopax3:entityFeature |
urn:biopax:ModificationFeature:XRCC6_HUMAN_1,
urn:biopax:ModificationFeature:XRCC6_HUMAN_10,
urn:biopax:ModificationFeature:XRCC6_HUMAN_11,
urn:biopax:ModificationFeature:XRCC6_HUMAN_12,
urn:biopax:ModificationFeature:XRCC6_HUMAN_2,
urn:biopax:ModificationFeature:XRCC6_HUMAN_3,
urn:biopax:ModificationFeature:XRCC6_HUMAN_4,
urn:biopax:ModificationFeature:XRCC6_HUMAN_5,
urn:biopax:ModificationFeature:XRCC6_HUMAN_6,
urn:biopax:ModificationFeature:XRCC6_HUMAN_7,
urn:biopax:ModificationFeature:XRCC6_HUMAN_8,
urn:biopax:ModificationFeature:XRCC6_HUMAN_9
|
| biopax3:organism | |
| biopax3:sequence |
MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD
|
| biopax3:standardName |
X-ray repair cross-complementing protein 6
|