| Predicate | Object |
|---|---|
| rdf:type | |
| biopax3:comment |
FUNCTION: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. CATALYTIC ACTIVITY: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. ENZYME REGULATION: Specifically inhibited by camptothecin (CPT), a plant alkaloid with antitumor activity. SUBUNIT: Monomer. Interacts with SV40 Large T antigen; this interactions allows viral DNA replication. SUBCELLULAR LOCATION: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. TISSUE SPECIFICITY: Endothelial cells. PTM: Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. DISEASE: Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. MISCELLANEOUS: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils. SIMILARITY: Belongs to the type IB topoisomerase family. SEQUENCE CAUTION: Sequence=CAA36834.1; Type=Erroneous gene model prediction; WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/TOP1ID320ch20q11.html"; GENE SYNONYMS:TOP1. COPYRIGHT: Protein annotation is derived from the UniProt Consortium (http://www.uniprot.org/). Distributed under the Creative Commons Attribution-NoDerivs License.,
SEQUENCE 765 AA; 90726 MW; 6FBED540BCF7BE28 CRC64;
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| biopax3:xref |
urn:biopax:RelationshipXref:HGNC_HGNC:11986,
urn:biopax:RelationshipXref:NCBI GENE_7150,
urn:biopax:RelationshipXref:REFSEQ_NP_003277,
urn:biopax:UnificationXref:UNIPROT_A8KA78,
urn:biopax:UnificationXref:UNIPROT_E1P5W3,
urn:biopax:UnificationXref:UNIPROT_O43256,
urn:biopax:UnificationXref:UNIPROT_P11387,
urn:biopax:UnificationXref:UNIPROT_Q12855,
urn:biopax:UnificationXref:UNIPROT_Q12856,
urn:biopax:UnificationXref:UNIPROT_Q15610,
urn:biopax:UnificationXref:UNIPROT_Q5TFY3,
urn:biopax:UnificationXref:UNIPROT_Q9UJN0
|
| biopax3:displayName |
TOP1_HUMAN
|
| biopax3:name |
5.99.1.2,
DNA topoisomerase I,
TOP1
|
| biopax3:entityFeature | |
| biopax3:organism | |
| biopax3:sequence |
MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDAKIKKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPRDEDDADYKPKKIKTEDTKKEKKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF
|
| biopax3:standardName |
DNA topoisomerase 1
|