_7771582912487198814

2007 publication year MI:0886, 9 last-imex-assigned, A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes., Apoptosis - Interactions involving proteins with a function related to apoptosis dataset MI:0875, Gloeckner CJ., Boldt K., Schumacher A., Roepman R., Ueffing M. author-list MI:0636, In this experiment purified proteins were sperated using 4-12% NuPAGE gradient gels (Invitrogen), stained and excised from the gel prior to Mass Spectrophotometric based identification. comment MI:0612, Only protein-protein interactions full coverage MI:0957, Proteomics (1615-9853) journal MI:0885, Synapse - Interactions of proteins with an established role in the presynapse. dataset MI:0875, The authors used a modified TAP tagging protocol. The TAP tag consisted of the Strep tag (WSHPQFEK) and a single FLAG tag. Unlike conventional TAP tagging constructs the TEV cleavage site was ommited. exp-modification MI:0627, imex curation MI:0959, imex curation curation depth MI:0955, marius.ueffing@gsf.de contact-email MI:0634

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