_7023087521227090901

2003 publication year MI:0886, A mamalian two hybrid system was used in this study. In this system, one protein is fused to the GAL4 DNA-BD and the other to the VP16 AD. Transcription from a GAL4 promoter upstream from a firefly luciferase reporter gene is activated when the BD and AD containing proteins are brought into close proximity, with interaction quantified by a luciferase activity assay. exp-modification MI:0627, DISC1 (Disrupted-In-Schizophrenia 1) is a centrosome-associated protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL: regulation and loss of interaction with mutation., Figure 3. Figure 8 (I). figure legend MI:0599, Hum. Mol. Genet. (0964-6906) journal MI:0885, Human heart cDNA library library-used MI:0672, Morris JA., Kandpal G., Ma L., Austin CP. author-list MI:0636, NCBI accession numbers were mapped to UniProt accession numbers using MPSRCH tool. data-processing MI:0633, Owing to the large number of positives, colonies were replated in high stringency medium (minus ade, his, leu, trp and Xgal), which reduced the number of positive colonies to 19 from the brain library and 40 from the heart library. exp-modification MI:0627, Synapse - Interactions of proteins with an established role in the presynapse. dataset MI:0875, Table 1 figure legend MI:0599, austin@mail.nih.gov contact-email MI:0634

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