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2007 publication year MI:0886, Apoptosis - Interactions involving proteins with a function related to apoptosis dataset MI:0875, Cancer - Interactions investigated in the context of cancer dataset MI:0875, LnCAP cells were androgen-stimulated (treated with 10 nM R1881 for 24 hours). Coimmunoprecipitation was performed on LnCAP cell lysates with anti-NDRG1 antibody-coated beads. The beads were boiled with SDS sample buffer and analyzed by SDS-PAGE. Proteins were detected by silver staining and in-gel trypsin digestion was carried out. Peptides were analyzed by high pressure liquid chromatography nanoelectrospray tandem mass spectrometry on a LTQ mass spectrometer (Thermo Electron). exp-modification MI:0627, Mol. Cell Proteomics (1535-9476) journal MI:0885, Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells., The authors in answer to a query replied that they used as reference sequences ipi.HUMAN.v3.17 (May 2006). I used Align to check similarity with the UniProtKb sequences with the referenced IPI sequences and added ranges and short cautions as appropriate when there were differences. Some of the sequences from v3.17 are not the current versions and then Uniparc searches were used to find the version of the IPI sequence current in May 2006. data-processing MI:0633, Tu LC., Yan X., Hood L., Lin B. author-list MI:0636, blin@systemsbiology.org contact-email MI:0634
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