The death receptor 5 (DR-5), a receptor for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), is critical for TRAIL-mediated apoptosis in various tumor cells. The ESE-3, a member of Ets transcription factors, regulates the expression of a variety of cellular genes by binding to purine-rich GGAA/T core sequence in cooperation with other transcription factors and co-factors. In this study, we demonstrate for the first time that ESE-3 regulates DR-5 expression through Ets binding sequences on the DR-5 promoter. Using a combination of the electrophoretic mobility shift assay and the luciferase reporter assay, we identified putative Ets sites responsible for ESE-3 transcriptional activity on the DR-5 promoter. In addition, we show the possible involvement of co-factors CBP and p300 in ESE-3-mediated DR-5 up-regulation.
| Subject | Predicate | Object | Context |
|---|---|---|---|
| http://purl.uniprot.org/cit... | rdf:type | uniprot:Journal_Citation | lld:uniprot |
| http://purl.uniprot.org/cit... | rdfs:comment | The death receptor 5 (DR-5), a receptor for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), is critical for TRAIL-mediated apoptosis in various tumor cells. The ESE-3, a member of Ets transcription factors, regulates the expression of a variety of cellular genes by binding to purine-rich GGAA/T core sequence in cooperation with other transcription factors and co-factors. In this study, we demonstrate for the first time that ESE-3 regulates DR-5 expression through Ets binding sequences on the DR-5 promoter. Using a combination of the electrophoretic mobility shift assay and the luciferase reporter assay, we identified putative Ets sites responsible for ESE-3 transcriptional activity on the DR-5 promoter. In addition, we show the possible involvement of co-factors CBP and p300 in ESE-3-mediated DR-5 up-regulation. | lld:uniprot |
| http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:name | Biochem. Biophys. Res. Commun. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Park Y.B. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Kwon T.K. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Lim J.H. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Park J.-W. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:author | Cho J.-Y. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:date | 2006 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:pages | 736-741 | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:title | ESE-3 transcription factor is involved in the expression of death receptor (DR)-5 through putative Ets sites. | lld:uniprot |
| http://purl.uniprot.org/cit... | uniprot:volume | 350 | lld:uniprot |
| http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1016/j.bbrc.2006.09.102 | lld:uniprot |
| uniprot-protein:Q9NZC4 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
| http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
| http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |