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skos:definition |
MSH: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.,CSP: mutagenesis where the mutation is caused by in vitro induction directed at a specific site in a DNA molecule; the most common method involves use of a chemically synthesized oligonucleotide mutant which can hybridize with the DNA target molecule; the resulting mismatch-carrying DNA duplex may then be transfected into a bacterial cell line and the mutant strands recovered.,NCI: A mutation created in a gene at a specific location. Targeted mutations are frequently generated in the laboratory to investigate the influence of inactivation of a particular gene product.,NCI: Any of several methods used to create alterations in a gene at a specific location.
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