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skos:definition |
MSH: DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.,NCI: Based upon the natural F plasmid of E. coli. Inserts up to 300 Kb can be cloned and stably maintained as circular molecules.
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