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skos:definition |
MSH: A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.,NCI: A method of detecting sequence differences between normal DNA and the DNA to be tested. It is commonly used as a screening method to detect potential mutations in a gene.
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